Shuttle Box Active Avoidance FR-2 Protocol

Summary

• Animal has to cross from one side, to the other, then back again
  • After activating the conditioned stimulus, aversive stimulation is activated on both sides of the chamber
  • Animal has to cross two times to deactivate it
• Each session is automatically divided into five blocks:
  • Acclimation period
  • FR-1 trials
  • Observation period
  • FR-1 trials
  • FR-2 trials
  • NOTE: Durations are user defined and can be eliminated as needed
• Stimuli presentation (5 sec) followed by an aversive stimulus escape interval (25 sec max)
• Mean Inter-Trial Interval (ITI) = 15 sec
• Maximum session time = 60 minutes
• User defined:
  • Stimuli
  • Avoid times
  • Escape times
  • CS/UCS overlap
  • Session time
  • ITI crossings punishment
• Data collected from each trial includes:
  • Trial count
  • FR value
  • Avoid counts & latency
  • Escape counts & latency
  • Left & right movements
  • Crossings
  • ITI aversive stimulations

SKU:   SOF-700RA-23